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Our results indicated that neither GHK nor GHK-Cu showed cytotoxic activity at the range of concentrations tested while CuCl 2 and Cu(OAc) 2 showed substantial cytotoxicity to the HaCaT cells at the highest concentration assayed, i.e., at 5800 M, after 30 mins treatment

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Cells were first stained with 50 M DCFDA or 10 M mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences

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Glutathione , often referred to as the master antioxidant, is a tripeptide molecule naturally produced within our cells